This page shows a list of allusions to the franchise of the Equestria Girls series and generations, works of fiction, people, places, events, and other cultural touchstones in the series My Little Pony Equestria Girls.
Re-use of sound assets
- The famous Wilhelm scream is used in My Little Pony Equestria Girls when Sunset Shimmer starts flying toward the crowd behind the gap she broke in the front of Canterlot High School, and in Player Piano when one of the students is pushed by the grand piano.
- Fluttershy's nervous squeal is used in My Little Pony Equestria Girls when she gives out fliers to help the animal shelter.
- The main cast's laugh is used in My Little Pony Equestria Girls, shortly after the conclusion of the reprise of This is Our Big Night.
- Rainbow Dash's exclamation of "So awesome!" is used in My Little Pony Equestria Girls when Twilight Sparkle refuses to hand the element of magic over to Sunset Shimmer.
- The sound used for Fluttershy's grin is used in Rainbow Rocks.
- Segments of the Cutie Mark Crusaders theme song are from Friendship is Magic.
- Rarity's gasp is used in Rainbow Rocks when she gasps at her spilt nail polish.
- Twilight's line "Never mind. It's not important." is used in Rainbow Rocks.
- Pinkie Pie's line when referring to Trixie's exit, "She's gone! Oh, wait, there she is," is used twice in Rainbow Rocks.
- Rarity's scoffing sound from Equestria Girls is reused once in Friendship Games.
- When Twilight Sparkle and Fluttershy introduce themselves, they pay homage to when Twilight Sparkle first meets Fluttershy's pony counterpart.
- The transforming sound effect from The Transformers is used when Pinkie Pie uncurls from being a ball at the start of the film.
- When Twilight Sparkle and Spike see the person walking his dog after Twilight first arrives in the other world, the dog is drawn in the same style as the 2010 version of Pound Puppies.
- Fluttershy describes all the different student cliques to Twilight while they're in the cafeteria, referencing a scene from Mean Girls.
- The skull that “Watermelody” is holding is a reference to the character Yorick from William Shakespeare's Hamlet.
- Sunset Shimmer comments to Twilight Sparkle about Spike, "and your little dog too," referencing The Wizard of Oz.
- In the part where the Crusaders are looking at their music video, there is a brown cat wearing a bow in the related videos that bears a striking resemblance to Fluffy from Pound Puppies. Also, the website that the Crusaders are surfing resembles the video-sharing website YouTube.
- During Twilight and Rainbow Dash’s one-on-one soccer match, the music is similar to "A Little Less Conversation" by Elvis Presley.
- During Time to Come Together, when Twilight tosses brooms to the rest of the main cast, Fluttershy flinches and lets the broom hit her instead. This is a reference to a scene from Arrested Development.
- In the first Fall Formal dance scene, Sweetie Belle dances similarly to Tsukasa Hiiragi in the opening sequence of Lucky Star. 
- When the spell is lifted from the Canterlot High School students, removing them from Sunset Shimmer's mind control, the beam travels through them all like the power of the Ark of the Covenant did to the German soldiers in Raiders of the Lost Ark.
- In the final dance of the film, near Trixie, Micro Chips is doing Psy's signature "invisible horse dance" from Gangnam Style. 
- Scootaloo does the Chicken Dance. This is a reference to the Friendship is Magic episode Stare Master, where she is called a chicken by Apple Bloom.
- The Dazzlings were inspired by the sirens from Greek mythology.
- The video game controllers that Applejack and Rainbow Dash play with resemble those of the Nintendo GameCube system.
- One of the outfits Rarity models during the rehearsal is similar to the suits The Beatles wore on the cover of their 1967 Sgt. Pepper's Lonely Hearts Club Band album, an idea further suggested by Rarity calling it "old-fashioned."
- During the Battle of the Bands, Snails goes by the stage name "DJ Snazzy Snails," similar to DJ Jazzy Jeff.
- After Pinkie catches Trixie hiding the first time, Trixie escapes leaving some hairpins behind much like Witch Hazel from Looney Tunes.
- The arena where the final battle takes place looks similar to the Hollywood Bowl in Los Angeles, California, with a saddle replacing the dome.
- The fight between the Dazzlings and the Rainbooms near the finale is similar to the scene in Scott Pilgrim vs. the World in which Pilgrim's band faces off against the Katayanagi twins.
- Sci-Twi going through Crystal Prep's doors before the opening credits is a parody of the main title sequence from the 1995 TV series Get Smart.
- The filling of the winning cake during "ACADECA" is shaped like the famous Mona Lisa painting by Leonardo da Vinci.
- Applejack loosing her second arrow in slow motion (during the Friendship Games) is similar to when Merida looses her final arrow from the 2012 Disney animated film Brave.
- The song "Unleash the Magic" has a very similar sound and rhyme scheme to "Poor Unfortunate Souls" from the 1989 Disney animated film The Little Mermaid.
- After Twilight sees Sci-Twi, the entire orchestra becomes silent for two xylophone hits for an eyeblink. This was a trademark of Warner Bros. cartoon composer Carl Stalling.
- The scene of DJ Pon-3 walking and dancing down the street is a reference to the "Staying Alive" scene in the 1977 film Saturday Night Fever.
- One scene in the short depicts a dog alluding to Paris Hilton's pet that she carries around.
- The title is a reference to musical instrument store chain Guitar Center.
- During the Shred Off, several of the licks Rainbow Dash plays are similar to actual rock songs.
- There is a poster in the guitar store that features the logo of British rock band, The Who.
- The title is a play on the title of the 1979 movie Apocalypse Now.
- Fluttershy leading the hamsters into the habitat is an allusion to the legend of the Pied Piper of Hamelin.
- Rarity names one of the hamsters Emilia Furhart, a reference to famous aviatrix Amelia Earhart.
- Pinkie's drumming style is reminiscent of that of Animal from the Muppets.
- Pinke saying "Po-tay-to, to-mah-to" is a reference to the George Gershwin song "Let's Call the Whole Thing Off."
- The record albums on display are Pink Floyd's Dark Side of the Moon, The Velvet Underground's self-titled album, and Wilco's "'A.M.".
- The Rainbooms facial makeup displaying their cutie marks resembles the band KISS' facial makeup.
- The makeup is also reminiscent of the Hasbro TV series and property Jem and the Holograms.
- Granny Smith, "Blueberry Cake," and "Rose Heart" do Beyoncé's "Single Ladies" dance.
- In a photo showing Twilight, Pinkie Pie and Rarity having fun on the carousel, Pinkie Pie is shown riding a carousel horse version of herself.
- The shot of Sunset letting her leather jacket blow away in the wind is similar to the shot of Elsa letting her cape blow away in the wind in the 2013 film Frozen.
- Each of the Rainbooms’ attire as well as their respective backdrop represents a different music period: Twilight Sparkle in the 1950s, Fluttershy in the 1960s, Rarity in the 1970s, Rainbow Dash in the 1980s, Applejack in the 1990s, and Pinkie Pie in the 2000s.
- The background scenery shown during Rarity's verse is reminiscent of the 1968 animated film Yellow Submarine.
- Rainbow Dash smashes her guitar at the end of her verse, which was a trademark of Pete Townshend of The Who.
- The scene of the Rainbooms singing together on a darkened background is a reference to Queen's "Bohemian Rhapsody" music video.
- The scene where Sunset Shimmer is singing while on a spinning record is a reference to the opening sequence of Happy Days.
- When Rarity gives Cheerilee a new look, she has pigtails tied with green flowers, like her G3.5 look.
- Once again, Rarity does Gene Kelly's lamppost dance from Singin’ in the Rain.
- The title is a parody of the proverb "All is fair in love and war," which is often attributed to John Lyly’s Euphues.
- One brief scene of Lyra and Sweetie Drops facing each other before the contest mirrors the character placement and on-screen display of early arcade fighting games.
- When Lyra and Sweetie Drops face off, one shot is posed and staged almost identically to a shot in the 2013 anime television series Kill la Kill, during the fight sequence in episode 3 where the two characters are viewed in silhouette against a pure white background, as one combatant leans far back.
- The mirror that Celestia shows Sunset Shimmer is similar to the Mirror of Erised from Harry Potter.
- Book titles on page 5 make a number of references to the TV series Fringe:
- Other book titles on the same page make reference to various TV series, movies, and comic book serials:
- In the final panel of page 8, the flyer by Sunset Shimmer's foot tells students to bring submissions to 'Megan, Danny or Molly', the main human characters of the classic My Little Pony television series.
- On page 36, one of the outfits Applejack tries on is the yellow and black striped jumpsuit made famous by Game of Death and Kill Bill.
- The cover appears to be based on the bottom panel on page 24 in Batman #686.
- The first panel is modeled after the introduction of every episode of The Powerpuff Girls, "The city of Townsville..."
- Pharaoh Phetlock is based on Victor Buono's depiction of King Tut from 1966's Batman television series.
- A ponified version of Commissioner James Gordon appears on page 6.
- Maretropolis' Mayor Blossom is named after Blossom of the Powerpuff Girls, and her mane and wardrobe are reminiscent of the Mayor from the same series.
- The name of Balkham Asylum is a play on DC Comics' Arkham Asylum.
- Hum Drum calling his friends jerks on page 27 is a reference to Uncanny X-Men issue #168 in which Kitty Pryde declares "Professor X is a jerk!"
- On page 31, the Mane-iac mentions a city called Trotham, a play on DC Comics' Gotham.
- The comic concludes with the narration "Same pony time, same pony channel," a reference to the Batman television series' closing episode narration.
- The social network MyStable is a parody of MySpace.
- On page 9, Fluttershy says "Use your limit break, Mr. Fuzzles!" "Limit break" is a video game term originating from Final Fantasy VII.
- On page 18, Rarity says to Applejack from the car, "Get in, AJ! We're going slumber partying!" This is a reference to a line delivered by actress Rachel McAdams in 2004's Mean Girls: "Get in, loser! We're going shopping!"
Equestria Girls: Through the Mirror
- Pinkie Pie makes mention of "Canterlot Idol," a reference to the reality television series American Idol.